Myosin lever arm orientation in muscle determined with high angular resolution using bifunctional spin labels
High-resolution structural information is invaluable for understanding muscle function. Savich et al. (https://bit.ly/2RxjOet) use bifunctional spin labeling to determine the orientation of the myosin lever arm in muscle fibers at high resolution under ambient conditions, augmenting previous insights obtained from fluorescence and EM.
Despite advances in x-ray crystallography, cryo-electron microscopy (cryo-EM), and fluorescence polarization, none of these techniques provide high-resolution structural information about the myosin light chain domain (LCD; lever arm) under ambient cond...