20/03/2024
Phytochemical, Proximate and Antimicrobial Screening in the aerial part plant of Drynaria sparsisora
Ogwuche Christiana Ene
Odogwu Chibuzor Blessing
Federal University of Petroleum Resources1
Keywords: Drynaira Sparsisora-Phytochemical-Antimicrobial-Proximate-Pathogens
Abstract
After being gathered from the Uviwe local government area in Delta state, the leaves of Drynaria sparsisora were mixed, air-dried, and then extracted using a soxhlet apparatus with n-hexane and methanol as the solvents. Using the standard procedures of the Association of Official Analytical Chemists (A.O.A.C.), the phytochemical and proximate analysis was performed on the extract. Results of the phytochemical screening carried out on the combined extracts (n-hexane and methanol) showed the presence of alkaloids, proteins, sugars, glycosides, tannins, phenolic compounds, flavonoids, and steroids. To ascertain the moisture content, carbon, lipid content, ash, fat, and nitrogen, proximate analysis was used. According to the results, there was 1.2% fat and 50.4% carbon in the sample. The following were obtained for the others: lipid content (34%), ash content (6.5%), and nitrogen (6.69%). Mueller Hinton agar medium was used to test the antimicrobial activities of both extracts against a variety of clinical pathogenic microorganisms, including Helico bacterpylori, Campylo bacterjejuni, Escherichia coli, Salmonel platypi, Proteus mirabilis, Candida albicans, Candida krusei, and Candida tropicalis. The zones of inhibition were determined, and at various concentrations, the n-hexane and methanol extract demonstrated resistance to Escherichia coli, vancomycin-resistant enterococci, methicillin-resistant Staph aureus, candida krusei, and candida tropicalis. Methanol and n-hexane extracts have MICs of 25 µg/m and 50 µg/m, respectively. For the extracts of methanol and n-hexane, the MBC/MFC are 50 µg/m and 25 µg/m, respectively.
https://www.journal.acse.science/index.php/ajce/article/view/169
