12/08/2025
How does the brain change as we wake up, grow tired, and finally sleep? An international research team led by the University of Michigan College of Literature, Science, and the Arts Department of Mathematics has created a new way to see it.
Using mouse models, the team combined:
• genetic tagging that lights up active neurons
• a cutting-edge imaging method called light-sheet microscopy
• new mathematical and computational analysis tools
Together, these techniques produced 3D, single-cell maps of which parts of the brain are active at different times of day.
The researchers observed that activity starts in deeper brain regions when mice wake, then shifts toward the cortex as wakefulness continues — a reorganization of brain networks that may hold clues to fatigue, sleep, and even mental health.
While the imaging method isn’t directly applicable to humans, the computational framework can be adapted for EEG, PET, and MRI data.
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