Journal of Cell Biology

Journal of Cell Biology The Journal of Cell Biology publishes peer-reviewed research on all aspects of cellular structure and function. Published by Rockefeller University Press.
The Journal of Cell Biology (JCB) features peer-reviewed research on all aspects of cellular structure and function. Areas include, but are not limited to: • Cell adhesion and motility • Cell cycle and division • Cell growth, survival, and death • Cell structure and dynamics • Cellular communication • Cellular disease mechanisms • Cytoskeleton and molecular motors • Gene expression and RNA metabolism • Methods and techniques • Nuclear organization, function, and structure • Organelle biogenesis and homeostasis • Protein, membrane, and lipid trafficking • Signal transduction • Stem cell biology • Systems and computational cell biology All editorial decisions are made by research-active scientists in conjunction with in-house scientific editors. JCB provides free online access to many article types immediately, with complete archival content freely available online. Established in 1955, JCB is published by The Rockefeller University Press. For more information, visit http://jcb.rupress.org. Follow us on Twitter: https://twitter.com/JCellBiol

On the cover of JCB—Image shows an “APPL tree.” Dual-color TIRF immunofluorescence images of APPL1 (green) and APP...
06/05/2019

On the cover of JCB—Image shows an “APPL tree.” Dual-color TIRF immunofluorescence images of APPL1 (green) and APPL2 (red) endosomes in H1975 cells after 20 ng/ml EGF stimulation. Image © Lakoduk et al., 2019. http://jcb.rupress.org/content/218/6/1928

Chen and colleagues find that the DNA damage repair protein XLF acts in concert with H2AX to prevent replication stress ...
06/05/2019
XLF and H2AX function in series to promote replication fork stability

Chen and colleagues find that the DNA damage repair protein XLF acts in concert with H2AX to prevent replication stress during DNA replication. https://bit.ly/2YKYOmY

XRCC4-like factor (XLF) is a non-homologous end joining (NHEJ) DNA double strand break repair protein. However, XLF deficiency leads to phenotypes in mice and humans that are not necessarily consistent with an isolated defect in NHEJ. Here we show that ...

The latest issue of JCB features new research on autophagosome biogenesis, mitochondrial diseases, axon growth inhibitio...
06/04/2019

The latest issue of JCB features new research on autophagosome biogenesis, mitochondrial diseases, axon growth inhibition, prostate cancer progression, Huntington disease, and more: https://bit.ly/2WdhYA0

Riggi et al. find that TORC2 impacts endocytosis through its effect on plasma membrane tension.  https://bit.ly/2JYx5ed
06/04/2019
TORC2 controls endocytosis through plasma membrane tension

Riggi et al. find that TORC2 impacts endocytosis through its effect on plasma membrane tension. https://bit.ly/2JYx5ed

Target of rapamycin complex 2 (TORC2) is a conserved protein kinase that regulates multiple plasma membrane (PM)–related processes, including endocytosis. Direct, chemical inhibition of TORC2 arrests endocytosis but with kinetics that is relatively slow...

Mondin et al. describe a phosphatase-independent function of the tumor suppressor protein PTEN in controlling endosomal ...
06/03/2019
PTEN reduces endosomal PtdIns(4,5)P2 in a phosphatase-independent manner via a PLC pathway

Mondin et al. describe a phosphatase-independent function of the tumor suppressor protein PTEN in controlling endosomal PtdIns(4,5)P2 levels. https://bit.ly/2X6RdPk

The tumor suppressor PTEN dephosphorylates PtdIns(3,4,5)P3 into PtdIns(4,5)P2. Here, we make the unexpected discovery that in Drosophila melanogaster PTEN reduces PtdIns(4,5)P2 levels on endosomes, independently of its phosphatase activity. This new PTE...

Our latest People & Ideas features Kota Saito, whose lab studies the role of TANGO1 proteins in cargo export from the en...
05/31/2019
Kota Saito: Getting out and about

Our latest People & Ideas features Kota Saito, whose lab studies the role of TANGO1 proteins in cargo export from the endoplasmic reticulum. Learn more about his research interests and views on succeeding in science here: https://bit.ly/2EwTgEO

Saito studies the mechanisms that control secretion of collagen from the ER.

Dhatchinamoorthy et al. describe the plastic nature of the outer kinetochore and identify spindle-proximal factors that ...
05/31/2019
The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors

Dhatchinamoorthy et al. describe the plastic nature of the outer kinetochore and identify spindle-proximal factors that influence its stoichiometry. https://bit.ly/2EpBRxZ

The kinetochore is a large molecular machine that attaches chromosomes to microtubules and facilitates chromosome segregation. The kinetochore includes submodules that associate with the centromeric DNA and submodules that attach to microtubules. Additi...

This Spotlight discusses work by Kalinski et al., (https://bit.ly/2YptQRa) who recently demonstrated that HDAC6 inhibiti...
05/30/2019
HDAC6 and Miro1: Another interaction causing trouble in neurons

This Spotlight discusses work by Kalinski et al., (https://bit.ly/2YptQRa) who recently demonstrated that HDAC6 inhibition prevents Miro1 deacetylation and allows for increased mitochondrial transport throughout axons and outgrowth into the surrounding extracellular matrix. https://bit.ly/2YLuI2A

Myelin-associated glycoprotein and chondroitin sulfate proteoglycans in the extracellular matrix can prevent regeneration of injured axons. In this issue, Kalinski et al. (2019. J. Cell Biol. ) report that inhibiti...

David et al. discover that Augmin-mediated growth of microtubules creates a directional bias towards kinetochores during...
05/30/2019
Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth

David et al. discover that Augmin-mediated growth of microtubules creates a directional bias towards kinetochores during metaphase. https://bit.ly/2WhBjnN

Dividing cells reorganize their microtubule cytoskeleton into a bipolar spindle, which moves one set of sister chromatids to each nascent daughter cell. Early spindle assembly models postulated that spindle pole–derived microtubules search the cytoplasm...

How macroH2A, a histone variant involved in silencing gene expression, is inherited from parent to daughter cells is unc...
05/29/2019
Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains

How macroH2A, a histone variant involved in silencing gene expression, is inherited from parent to daughter cells is unclear. Sato et al. (https://bit.ly/2QfbaRq) use live cell imaging, genomics and biochemistry to investigate how silencing information can be transmitted during the growth of cells, providing insight into epigenetic cellular memory.

The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance of cellular memory of prior states. The macroH2A histone variant has properties of a regulator of epigenetic cell memory, including roles...

Toivanen and Furic preview work from the Trotman laboratory (https://bit.ly/2LJbmcX) showing that the phosphatase PHLPP2...
05/28/2019
A balancing act: PHLPP2 fine tunes AKT activity and MYC stability in prostate cancer

Toivanen and Furic preview work from the Trotman laboratory (https://bit.ly/2LJbmcX) showing that the phosphatase PHLPP2 controls Myc stability, suggesting a new way to target Myc in prostate cancer. https://bit.ly/2w1B0yK

PTEN loss stimulates prostate tumor progression by sustaining AKT activation. Nowak et al. (2019. J. Cell Biol . ) surprisingly show that the AKT-suppressing phosphatase PHLPP2 promotes disease progression in the c...

Miteva et al. show that Rab46 integrates histamine signaling and Ca2+ signals to regulate selective cargo release from W...
05/24/2019
Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies

Miteva et al. show that Rab46 integrates histamine signaling and Ca2+ signals to regulate selective cargo release from Weibel-Palade bodies in endothelial cells. https://bit.ly/2Q4Dh5v

Endothelial cells selectively release cargo stored in Weibel-Palade bodies (WPBs) to regulate vascular function, but the underlying mechanisms are poorly understood. Here we show that histamine evokes the release of the proinflammatory ligand, P-selecti...

Zhao et al. identify more than 40 new potential components of the central apparatus of Chlamydomonas cilia. Many are con...
05/23/2019
Proteome of the central apparatus of a ciliary axoneme

Zhao et al. identify more than 40 new potential components of the central apparatus of Chlamydomonas cilia. Many are conserved and will facilitate genetic screening of patients with a form of primary ciliary dyskinesia that is difficult to diagnose. https://bit.ly/2Q21ae7

Nearly all motile cilia have a “9+2” axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate...

Nakajima et al. reveal a novel mechanism of planar spindle alignment through the junctional tumor suppressors Scrib/Dlg ...
05/22/2019
Junctional tumor suppressors interact with 14-3-3 proteins to control planar spindle alignment

Nakajima et al. reveal a novel mechanism of planar spindle alignment through the junctional tumor suppressors Scrib/Dlg and 14-3-3 proteins that maintains tissue architecture in the Drosophila wing disc epithelium. https://bit.ly/2W3UHEO

Proper orientation of the mitotic spindle is essential for cell fate determination, tissue morphogenesis, and homeostasis. During epithelial proliferation, planar spindle alignment ensures the maintenance of polarized tissue architecture, and aberrant s...

Zhang and Balachandran preview new findings from Sai et al. (https://bit.ly/2GhmoBt) revealing that necroptosis signalin...
05/21/2019
Bayonets over bombs: RIPK3 and MLKL restrict Listeria without triggering necroptosis

Zhang and Balachandran preview new findings from Sai et al. (https://bit.ly/2GhmoBt) revealing that necroptosis signaling mediators can directly inhibit Listeria replication in the gut epithelium https://bit.ly/2w0jNWn

RIPK3 induces necroptosis by phosphorylating MLKL, which then induces plasma membrane rupture and necrotic cell death. In this issue, Sai et al. (2019. J. Cell Biol. ) show that RIPK3-MLKL signaling in epithelial c...

Children with mitochondrial diseases often present with slow growth and short stature, but the underlying mechanism rema...
05/21/2019
Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases

Children with mitochondrial diseases often present with slow growth and short stature, but the underlying mechanism remains unclear. Holzer et al. provide in vivo evidence that mitochondrial respiratory chain dysfunction induces cartilage degeneration coincident with altered metabolism, impaired extracellular matrix formation, and cell death at the cartilage–bone junction. https://bit.ly/2W018IW

In childhood, skeletal growth is driven by transient expansion of cartilage in the growth plate. The common belief is that energy production in this hypoxic tissue mainly relies on anaerobic glycolysis and not on mitochondrial respiratory chain (RC) act...

Nowak et al. show that loss of the AKT-inactivating phosphatase PHLPP2 paradoxically blocks prostate tumor growth and me...
05/20/2019
The PHLPP2 phosphatase is a druggable driver of prostate cancer progression

Nowak et al. show that loss of the AKT-inactivating phosphatase PHLPP2 paradoxically blocks prostate tumor growth and metastasis. PHLPP2, they find, is critical for MYC stability, suggesting that PHLPP2 inhibitors may present a therapeutic opportunity to target MYC. https://bit.ly/2LJbmcX

Metastatic prostate cancer commonly presents with targeted, bi-allelic mutations of the PTEN and TP53 tumor suppressor genes. In contrast, however, most candidate tumor suppressors are part of large recurrent hemizygous deletions, such as the common chr...

Feng et al. describe persistent neuronal microtubule minus end growth that depends on the CAMSAP protein Patronin and is...
05/20/2019
Patronin-mediated minus end growth is required for dendritic microtubule polarity

Feng et al. describe persistent neuronal microtubule minus end growth that depends on the CAMSAP protein Patronin and is needed for dendritic minus-end-out polarity. https://bit.ly/2w1Gn0N

Microtubule minus ends are thought to be stable in cells. Surprisingly, in Drosophila and zebrafish neurons, we observed persistent minus end growth, with runs lasting over 10 min. In Drosophila , extended minus end growth depended on Patronin, and Patr...

A new JCB study (https://bit.ly/2LJbmcX) reveals that inhibiting PHLPP2 lowered the levels of MYC, an oncogenic protein ...
05/16/2019

A new JCB study (https://bit.ly/2LJbmcX) reveals that inhibiting PHLPP2 lowered the levels of MYC, an oncogenic protein that causes many different types of cancer that cannot be targeted by conventional drug therapies. Read the press release here: https://bit.ly/2w15VeD

We've harmonized our manuscript submission systems with the Journal of Cell Science (JCS) and Molecular Biology of the C...
05/16/2019

We've harmonized our manuscript submission systems with the Journal of Cell Science (JCS) and Molecular Biology of the Cell (MBoC) to reduce the burden on authors and reviewers. Read all about the new option here: https://bit.ly/2W5SwAH

Sharma and Subramaniam demonstrate that Rhes, a brain striatum-enriched protein, increases biogenesis of tunneling nanot...
05/16/2019
Rhes travels from cell to cell and transports Huntington disease protein via TNT-like protrusion

Sharma and Subramaniam demonstrate that Rhes, a brain striatum-enriched protein, increases biogenesis of tunneling nanotube-like cellular protrusions through which Rhes travels from cell to cell and transports Huntington disease protein. This may explain why Huntington's disease selectively affects striatal neurons. https://bit.ly/2WFnqwQ

Tunneling nanotubes (TNT) are thin, membranous, tunnel-like cell-to-cell connections, but the mechanisms underlying their biogenesis or functional role remains obscure. Here, we report, Rhes, a brain-enriched GTPase/SUMO E3-like protein, induces the bio...

There are ~60 Rab GTPases in mammals that act as master regulators of intracellular membrane trafficking. Homma et al. e...
05/15/2019
Comprehensive knockout analysis of the Rab family GTPases in epithelial cells

There are ~60 Rab GTPases in mammals that act as master regulators of intracellular membrane trafficking. Homma et al. establish a comprehensive collection of knockout epithelial cell lines for all mammalian Rabs, revealing that Rab6 is required for basement membrane formation and soluble cargo secretion. https://bit.ly/2Yt2vxE

The Rab family of small GTPases comprises the largest number of proteins (∼60 in mammals) among the regulators of intracellular membrane trafficking, but the precise function of many Rabs and the functional redundancy and diversity of Rabs remain largel...

New work from Kalinski et al. shows that central nervous system growth inhibitors attenuate axon growth by deacetylation...
05/14/2019
Deacetylation of Miro1 by HDAC6 blocks mitochondrial transport and mediates axon growth inhibition

New work from Kalinski et al. shows that central nervous system growth inhibitors attenuate axon growth by deacetylation of Miro1 through HDAC6, thereby blocking mitochondrial transport. https://bit.ly/2YptQRa

Inhibition of histone deacetylase 6 (HDAC6) was shown to support axon growth on the nonpermissive substrates myelin-associated glycoprotein (MAG) and chondroitin sulfate proteoglycans (CSPGs). Though HDAC6 deacetylates α-tubulin, we find that another HD...

Our latest issue features new research on autophagosomal membranes, invadosomes, neuromuscular synapses, cargo transport...
05/10/2019

Our latest issue features new research on autophagosomal membranes, invadosomes, neuromuscular synapses, cargo transport within the Golgi, and more: https://bit.ly/2DXz92l

On the cover of JCB—Electron micrograph image showing organization of sarcomeres in skeletal myofibers, including the ...
05/09/2019

On the cover of JCB—Electron micrograph image showing organization of sarcomeres in skeletal myofibers, including the triads formed by the juxtaposition of the sarcoplasmic reticulum and transverse tubules with adjacent mitochondria shown in blue. The I-bands, containing actin, and the A-bands, containing actin and myosin, are colored in red. Image © Oury et al. 2019 https://bit.ly/2VSS3Sf

On the cover of JCB—Electron micrograph image showing organization of sarcomeres in skeletal myofibers, including the ...
05/09/2019

On the cover of JCB—Electron micrograph image showing organization of sarcomeres in skeletal myofibers, including the triads formed by the juxtaposition of the sarcoplasmic reticulum and transverse tubules with adjacent mitochondria shown in blue. The I-bands, containing actin, and the A-bands, containing actin and myosin, are colored in red. Image © Oury et al. 2019 https://bit.ly/2VSS3Sf

Explore our collection of exciting new immunology, microbiology, and virology research.
05/08/2019
Special Collection: Immune cell biology

Explore our collection of exciting new immunology, microbiology, and virology research.

We are delighted to present this selection of cutting-edge research about immune cell biology, microbiology, and virology recently published in JCB.

In this special collection (https://bit.ly/2VQOtrG), JCB Editorial Board member Johanna Ivaska and Senior Scientific Edi...
05/07/2019
Special Collection: Cell Adhesion

In this special collection (https://bit.ly/2VQOtrG), JCB Editorial Board member Johanna Ivaska and Senior Scientific Editor Melina Casadio selected recent JCB content delving into the mechanisms of migration, cell–cell, and cell–matrix adhesions.

The adhesion machinery and interactions with the cellular microenvironment play essential roles in regulating cell function—including proliferation, migration, differentiation—in development and disease.

Lakoduk et al. reveal that mutant p53 upregulates dynamin-1 and recruitment of APPL1 to a subpopulation of endosomes, th...
05/06/2019
Mutant p53 amplifies a dynamin-1/APPL1 endosome feedback loop that regulates recycling and migration

Lakoduk et al. reveal that mutant p53 upregulates dynamin-1 and recruitment of APPL1 to a subpopulation of endosomes, thereby increasing receptor recycling and cell migration. https://bit.ly/2Y2fAxG

Multiple mechanisms contribute to cancer cell progression and metastatic activity, including changes in endocytic trafficking and signaling of cell surface receptors downstream of gain-of-function (GOF) mutant p53. We report that dynamin-1 (Dyn1) is up-...

Aksu et al. delineate the structures of central transport intermediates of the biportin Pdr6, including those involving ...
05/02/2019
Structural basis for the nuclear import and export functions of the biportin Pdr6/Kap122

Aksu et al. delineate the structures of central transport intermediates of the biportin Pdr6, including those involving Ubc9, RanGTP, and eIF5A. https://bit.ly/2IWBeiw (Find the accompanying paper from this group at https://bit.ly/2GJMH3t).

Importins ferry proteins into nuclei while exportins carry cargoes to the cytoplasm. In the accompanying paper in this issue (Vera Rodriguez et al. 2019. J. Cell Biol. ), we discovered that Pdr6 is a biportin that ...

Congratulations to JCB Editorial Board Member Rebecca Heald on her election to the National Academy of Sciences https://...
05/01/2019

Congratulations to JCB Editorial Board Member Rebecca Heald on her election to the National Academy of Sciences https://bit.ly/2Lg6agv

Rodriguez, Frey, & Gorlich develop a SUMO/protease system for use in eukaryotic systems. With this new tool, they find b...
05/01/2019
Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor

Rodriguez, Frey, & Gorlich develop a SUMO/protease system for use in eukaryotic systems. With this new tool, they find both nuclear import and export cargos for Pdr6. https://bit.ly/2GJMH3t (Find their companion paper at https://bit.ly/2IWBeiw )

Cleavage of affinity tags by specific proteases can be exploited for highly selective affinity chromatography. The SUMO/SENP1 system is the most efficient for such application but fails in eukaryotic expression because it cross-reacts with endogenous pr...

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The Journal of Cell Biology (JCB) features peer-reviewed research on all aspects of cellular structure and function. Areas include, but are not limited to: • Cell adhesion and motility • Cell cycle and division • Cell growth, survival, and death • Cell structure and dynamics • Cellular communication • Cellular disease mechanisms • Cytoskeleton and molecular motors • Gene expression and RNA metabolism • Methods and techniques • Nuclear organization, function, and structure • Organelle biogenesis and homeostasis • Protein, membrane, and lipid trafficking • Signal transduction • Stem cell biology • Systems and computational cell biology All editorial decisions are made by research-active scientists in conjunction with in-house scientific editors. JCB provides free online access to many article types from the date of publication and to all archival content. Established in 1955, JCB is published by The Rockefeller University Press. For more information, visit http://jcb.rupress.org. Follow us on Twitter: https://twitter.com/JCellBiol

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